Chromatin modifications in response to DNA double-strand breaks
DNA double-strand breaks (DSBs) are among the most toxic DNA lesions, and cells have to respond to DSBs quickly and in an efficient way in order to make sure that they are properly repaired. The response to DSBs involves modifications of the surrounding chromatin to generate dedicated repair domains. To mount these modifications in a robust and self-reinforcing manner, cells employ feed-forward mechanism. This provides a robust chromatin response beneficial for DSB repair, yet it comes with the risk that excessive spreading of DNA break-induced chromatin modifications may also affect undamaged areas of the genome. Indeed, we recently discovered that self-amplificatory chromatin transactions must be tightly controlled and actively restrained to remain locally and temporally confined, and one research focus of the lab lies on the cellular mechanisms that prevent excessive and potentially detrimental chromatin modifications in response to DNA breakage.
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Pellegrino & Altmeyer
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Altmeyer & Lukas
Guarding against collateral damage during chromatin transactions